Open AccessGlutamine synthetase from Acetobacter diazotrophicus : properties and regulation
Author(s) -
Ureta Alejandro,
Nordlund Stefan
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10800.x
Subject(s) - adenylylation , glutamine synthetase , biochemistry , enzyme , glutamine , biology , biosynthesis , diazotroph , nitrogen fixation , chemistry , microbiology and biotechnology , bacteria , amino acid , genetics
Glutamine synthetase from Acetobacter diazotrophicus , an endophyte originally isolated from sugarcane, was studied as a step in the identification of mechanisms underlying the role of A. diazotrophicus as a major supplier of fixed nitrogen to its host plant. The enzyme was purified and partially characterized. It was also shown that the enzyme is regulated by adenylylation in response to the nitrogen source. Interestingly, there is no upregulation of the synthesis of the enzyme under diazotrophic conditions, which is in contrast to the situation in enterics, e.g. Klebsiella pneumoniae .