Open AccessThe Aspergillus nidulans carnitine carrier encoded by the acuH gene is exclusively located in the mitochondria
Author(s) -
Ramón De Lucas J.,
Martı́nez Oscar,
Pérez Patricia,
Isabel López M.,
Valenciano Susana,
Laborda Fernando
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10756.x
Subject(s) - aspergillus nidulans , polyclonal antibodies , carnitine , escherichia coli , biology , peroxisome , biochemistry , gene , mitochondrion , blot , microbiology and biotechnology , affinity chromatography , gene product , chemistry , gene expression , antibody , genetics , enzyme , mutant
The location of the Aspergillus nidulans carnitine/acyl‐carnitine carrier (ACUH) was studied. ACUH with a His‐tag at its N‐terminus was over‐expressed in Escherichia coli and purified by Ni 2+ affinity chromatography. The purified protein was utilised to raise polyclonal antibodies which were characterised by Western blotting. For localisation studies A. nidulans T1 strain, that contains the acuH gene under control of the strong promoter alcA p , was derived. Results obtained demonstrate the exclusively mitochondrial localisation of ACUH and therefore exclude the targeting of the acuH gene product to the peroxisomal membrane.