Open AccessExpression of exo‐polygalacturonases in Botrytis cinerea
Author(s) -
Rha Eugene,
Park Hong Jai,
Kim Myeong Ok,
Chung Young Ryun,
Lee ChangWon,
Kim Jae Won
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10740.x
Subject(s) - botrytis cinerea , polyclonal antibodies , biology , botrytis , microbiology and biotechnology , pectin , pectinesterase , inoculation , enzyme , pectinase , spore , fungi imperfecti , pathogenic fungus , pathogenesis related protein , pathogen , fungus , antibody , biochemistry , gene expression , botany , gene , horticulture , immunology
The pathogenic fungus, Botrytis cinerea , causing gray mold disease in a variety of plant species, secretes at least four polygalacturonases (PGs), cell wall degrading enzymes. Among them, we prepared polyclonal antibody against purified 66‐kDa exo‐PG in rabbit. Immunoblot analysis revealed that the antibody recognized two exo‐PGs, 66 kDa and 70 kDa in molecular mass, secreted from B. cinerea cultured in the medium containing citrus pectin as a carbon source. By immunohistochemical analysis, the expression of exo‐PGs was identified in cucumber leaves inoculated with spores of B. cinerea . The exo‐PGs were observed 9 h after inoculation, and the amount of exo‐PGs increased with time in the leaves. The exo‐PGs were induced by polygalacturonic acid as well as its monomer, galacturonic acid, in vitro. The expression of 66‐kDa exo‐PG (exo‐PG I) increased with time of culture, while 70‐kDa exo‐PG (exo‐PG II) was transiently expressed soon after the start of culture. Therefore, exo‐PGs might play an important role in pathogenesis at an early stage of infection as well as in tissue maceration of host plant.