Open AccessNovel alcohol dehydrogenase activity in a mutant of Salmonella able to use ethanol as sole carbon source
Author(s) -
Dailly Yves,
MatJan Fairoz,
Clark David P
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10730.x
Subject(s) - alcohol dehydrogenase , aldehyde dehydrogenase , salmonella enterica , mutant , chemistry , ethanol , biochemistry , acetaldehyde , alcohol , escherichia coli , fermentation , isocitrate dehydrogenase , enzyme , microbiology and biotechnology , biology , gene
We selected a mutant of Salmonella enterica serovar Typhimurium that is capable of growing in air on ethanol as sole carbon and energy source. This adhI mutant expressed high levels of a novel alcohol dehydrogenase (AdhI) that uses ethanol, 1‐propanol and 2‐propanol as substrates. The fermentative AdhE alcohol dehydrogenase was not expressed aerobically in the adhI mutant. Anaerobically, both the novel AdhI enzyme and the AdhE were expressed simultaneously in the adhI mutant. However, the adhI mutant showed no alteration in the composition of the fermentation products. In addition we found that both the parental Salmonella and its alcohol using adhI mutant expressed substantial levels of a dye‐linked aldehyde dehydrogenase that is presumably responsible for conversion of acetaldehyde to acetate. This contrasts with the situation in Escherichia coli where mutants able to grow on ethanol express high aerobic levels of the AdhE enzyme, which performs both the alcohol dehydrogenase and aldehyde dehydrogenase reactions.