Open AccessIdentification of a cadmium‐induced gene in Mycobacterium bovis and Mycobacterium tuberculosis
Author(s) -
Hotter Grant S,
Wilson Theresa,
Collins Desmond M
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10707.x
Subject(s) - mycobacterium bovis , mycobacterium tuberculosis , gene , biology , tuberculosis , microbiology and biotechnology , promoter , mycobacterium , insertion sequence , genome , gene expression , genetics , bacteria , transposable element , pathology , medicine
A 17‐kDa protein (CadI) was induced by cadmium in Mycobacterium bovis and Mycobacterium tuberculosis . Comparison of the N‐terminal sequence from M. bovis CadI with the annotated M. tuberculosis genome database identified Rv2641 as the encoding gene. Long and short promoter fragments from M. bovis cadI were fused to the lacZ reporter gene in pYUB76. Only the long fragment directed cadmium‐inducible activity when electroporated into M. bovis . The cadI promoter has potential for both constitutive and inducible expression studies in M. bovis and M. tuberculosis .