Open AccessExpression profiles of low molecular mass heat shock proteins by Mycobacterium avium and Mycobacterium intracellulare
Author(s) -
Tomioka Haruaki,
Shimizu Toshiaki,
Kajitani Hiroko,
Sato Katsumasa,
Sano Chiaki
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10704.x
Subject(s) - heat shock protein , molecular mass , lysis , shock (circulatory) , gel electrophoresis , polyacrylamide gel electrophoresis , heat shock , hsp70 , mycobacterium , biology , microbiology and biotechnology , protein biosynthesis , chemistry , bacteria , biochemistry , medicine , enzyme , gene , genetics
Abstract Closely related non‐tuberculous mycobacterial species, Mycobacterium avium and Mycobacterium intracellulare , were compared for the profiles of their production of low molecular mass heat shock proteins at 45°C, by performing polyacrylamide gel electrophoresis analysis of bacterial cell lysate proteins. All of the M. intracellulare but not M. avium strains potently increased the production of the 18‐kDa heat shock protein, when cultured at 45°C. Half of the M. intracellulare strains with high sensitivity to 45°C produced not only the 18‐kDa heat shock protein but also the 16‐kDa heat shock protein at 45°C. These findings indicate that M. avium and M. intracellulare differentially respond to 45°C heat shock in terms of the production of low molecular mass heat shock proteins.