Open AccessDescription and application of a rapid method for genomic DNA direct sequencing
Author(s) -
Krin Evelyne,
Hommais Florence,
Soutourina Olga,
Ngo Saravuth,
Danchin Antoine,
Bertin Philippe
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10679.x
Subject(s) - biology , genomic dna , photorhabdus luminescens , vibrio cholerae , genetics , dna sequencing , dna , genome , dna nanoball sequencing , bacillus subtilis , computational biology , genomic library , gene , base sequence , bacteria
We describe a rapid method for determining nucleotide sequences directly from total genomic DNA. This technique was used to determine genomic DNA sequences in various prokaryotic and eukaryotic microorganisms with a G+C content between 40 and 50%, e.g. Escherichia coli , Vibrio cholerae , Bacillus subtilis and Saccharomyces cerevisiae . Furthermore, the method was applied to accurately sequence up to 300 DNA base pairs in Photorhabdus luminescens , whose genome sequencing is currently under way. Taken together, these results provide evidence that our technique can be widely used to easily and efficiently determine genomic DNA sequences.