Open AccessGenetic variability among archival cultures of Salmonella typhimurium
Author(s) -
Edwards Kelly,
Linetsky Irina,
Hueser Christopher,
Eisenstark Abraham
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10677.x
Subject(s) - pulsed field gel electrophoresis , salmonella , biology , gel electrophoresis , auxotrophy , agar , vial , polyacrylamide gel electrophoresis , population , genetics , microbiology and biotechnology , genotype , escherichia coli , bacteria , gene , chemistry , chromatography , biochemistry , enzyme , demography , sociology
The existence in our laboratory of over 10 000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33–46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10 3 –10 5 colony forming units per vial were recovered (less than 0.01% of the original population) even after decades of undisturbed storage. Considerable genetic variability was observed in these populations. Three genetic variables, chromosome fragment size as determined by pulsed‐field gel electrophoresis, extensive mutational reversions from nutritional auxotrophy to prototrophy, and differences in protein content as assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were measured.