Open AccessThe possible function of the glucan sheath of Botrytis cinerea : effects on the distribution of enzyme activities
Author(s) -
Gilad Nir L,
BarNun Nurit,
Mayer Alfred M
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10659.x
Subject(s) - mycelium , botrytis cinerea , catalase , glucan , peroxidase , microbiology and biotechnology , fungus , guaiacol , enzyme , hericium erinaceus , biology , enzyme assay , beta glucan , laccase , biochemistry , chemistry , botany , ecology , raw material
The effect of a β‐1,3‐glucanase (Glucanex) on cultures of Botrytis cinerea was examined. The enzyme released reducing sugars from the mycelium and from the glucan secreted into the culture medium. The morphology of the mycelium was changed in the presence of Glucanex. The measured activity of guaiacol peroxidase, laccase, and catalase was increased when the mycelium was treated with Glucanex. Culture of the mycelium in the presence of Glucanex resulted in an increase in catalase activity. We suggest that the glucan plays a role in protecting the fungus from host response and may assist in the initial stages of host infection.