Open AccessSeparation of 6‐deoxy‐heptane from a smooth‐type lipopolysaccharide preparation of Burkholderia pseudomallei
Author(s) -
Isshiki Yasunori,
Matsuura Motohiro,
Dejsirilert Surang,
Ezaki Takayuki,
Kawahara Kazuyoshi
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10645.x
Subject(s) - burkholderia pseudomallei , microbiology and biotechnology , melioidosis , burkholderia , heptane , lipopolysaccharide , bacteria , chemistry , chromatography , biology , immunology , organic chemistry , genetics
Smooth‐type lipopolysaccharide (LPS) of Burkholderia pseudomallei has been reported to contain two kinds of O‐antigenic polysaccharides, a 1,3‐linked homopolymer of 6‐deoxy‐heptose and a polymer with a repeating unit of →3)‐glucose‐(1→3)‐6‐deoxy‐talose‐(1→ with O ‐acetyl or O ‐methyl modifications. A LPS preparation containing these two polysaccharides was separated by gel‐permeation chromatography in this study. Chemical analysis of the separated fractions revealed the 6‐deoxy‐heptane to be a polysaccharide without a lipid portion and the polymer of glucose and 6‐deoxy‐talose to be an O‐antigenic polysaccharide of the LPS. This result was further supported by the assay of these polysaccharide molecules for macrophage activation activity. The 6‐deoxy‐heptane showed no macrophage activation, indicating that this polysaccharide was not the LPS, but one of the capsular polysaccharides of B. pseudomallei .