Open AccessCbbR, a LysR‐type transcriptional regulator from Hydrogenophilus thermoluteolus , binds two cbb promoter regions
Author(s) -
Terazono Koichi,
Hayashi Nobuhiro R,
Igarashi Yasuo
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10635.x
Subject(s) - transcriptional regulation , promoter , transcription (linguistics) , electrophoretic mobility shift assay , gene , dna , genetics , regulator , plasmid , transcription factor , biology , microbiology and biotechnology , gene expression , linguistics , philosophy
The cbbR encoding the LysR‐type transcriptional regulator is located downstream of cbbLSQOYA and this gene is located upstream of cbbFPT in divergent transcription. The two promoter regions with LysR‐binding sites are located in the cbbL upstream region and in the cbbR – cbbF intergenic region. Electrophoretic mobility shift assays using a cell extract of Escherichia coli harboring a plasmid containing cbbR and the DNA fragments of promoter regions indicated that CbbR binds in both regions. NADPH caused differences in the complex of CbbR and DNA.