Open AccessStructures of ribonuclease P RNAs of Vibrio core species
Author(s) -
Maeda Toshimichi,
Furushita Manabu,
Hamamura Kazuhiro,
Shiba Tsuneo
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10633.x
Subject(s) - biology , vibrio , vibrio alginolyticus , rnase p , rna , ribonuclease , genetics , escherichia coli , vibrio harveyi , ribonuclease iii , gene , microbiology and biotechnology , bacteria , rna interference
The structures of an RNA component of ribonuclease P (RNase P RNA) were examined for Vibrio parahaemolyticus , Vibrio alginolyticus , Vibrio carchariae , Vibrio natriegens , Vibrio campbellii , Vibrio proteolyticus , Vibrio pelagius and Vibrio harveyi to clearly determine their genetic differences. The RNase P RNAs ranged from 382 to 454 nucleotides (nt) in size, and were remarkably different from each other in the structure of two helices, P3 and P12. The P3 helices were comprised of tandem repeats of a palindromic sequence (24 nt), resulting in the longitudinal repetition of a stem structure. The number of repetitions ranged from four in V. harveyi , to one in both V. alginolyticus and V. proteolyticus . The genes for the RNase P RNAs of all species were located between two open reading frames, the amino acid sequences of which were similar to the hypothetical proteins located at 70.92 and 1.94 min in the Escherichia coli chromosome.