Open AccessUse of repetitive DNA elements to define genetic relationships among Anaplasma marginale isolates
Author(s) -
Ferreira Alda Maria T.,
Suzart Sérgio,
Vidotto Odilon,
Knowles Don P.,
Vidotto Marilda C.
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10595.x
Subject(s) - biology , intergenic region , palindrome , polymerase chain reaction , genetics , strain (injury) , rickettsiales , genomic dna , dna profiling , genetic diversity , dna , genome , gene , anatomy , population , demography , sociology
Anaplasma marginale genomic DNA was tested for the presence of repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC)‐like sequences in order to evaluate the genetic diversity of multiple A. marginale isolates. A. marginale isolates were obtained from cattle of six different states of Brazil, from the US and an Anaplasma centrale strain was obtained from Uruguay. Patterns obtained from A. marginale isolates varied from 14 to 17 fragments by REP‐polymerase chain reaction (PCR) and 6 to 14 fragments by ERIC‐PCR. All A. marginale isolates presented a 0.75‐kb fragment by REP and two common fragments (0.38 and 1.0 kb) by ERIC‐PCR. These two fragments were not detectable in A. centrale . Both methods produced similar patterns (80%) among A. marginale isolates obtained from the same region, although some isolates within regions shared less similarity. Isolates from Parana and Pernambuco, were differentiated by these methods. The study demonstrates the presence of ERIC and REP‐like elements in A. marginale isolates and shows that A. marginale isolates and strains can be differentiated by these methods.