Open AccessFlhD/FlhC‐regulated promoters analyzed by gene array and lacZ gene fusions
Author(s) -
Prüß Birgit M,
Liu Xiaojin,
Hendrickson William,
Matsumura Philip
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10588.x
Subject(s) - reductase , escherichia coli , nitrite reductase , biology , nitrate reductase , biochemistry , gene , microbiology and biotechnology , periplasmic space , enzyme
The Escherichia coli transcriptional regulatory complex FlhD/FlhC, initially identified as a flagella‐specific activator, is a global regulator involved in many cellular processes. Using gene arrays, lacZ gene fusions and enzyme assays, eight new targets of FlhD/FlhC were recognized. These are the transporter for galactose (MglBAC), the rod‐shape determination proteins (MreBCD), malate dehydrogenase, and several enzymes involved in anaerobic respiration (glycerol 3‐phosphate dehydrogenase, GlpABC; periplasmic nitrate reductase, NapFAGHBC; nitrite reductase, NrfABCDEFG; dimethyl sulfoxide reductase, DmsABC; and the modulator for hydrogenases, HydNHypF).