Open AccessMolecular cloning, nucleotide sequence and structural analysis of the Streptomyces galbus DSM40480 fda gene: the S. galbus fructose‐1,6‐bisphosphate aldolase is a member of the class II aldolases
Author(s) -
Wehmeier Udo F
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10582.x
Subject(s) - gene , aldolase a , biology , mutant , nucleic acid sequence , streptomyces , molecular cloning , genetics , microbiology and biotechnology , escherichia coli , sequence analysis , fructose bisphosphate aldolase , aldolase b , cloning (programming) , peptide sequence , biochemistry , bacteria , enzyme , computer science , programming language
The fda gene of Streptomyces galbus DSM40480 encoding the fructose‐1,6‐bisphosphate aldolase (EC 4.1.2.13) was cloned, sequenced and characterised. The fda gene encodes a protein of 341 amino acids with a molecular mass of 36.5 kDa and belongs to the class II aldolases. When the S. galbus fda gene was expressed in the Escherichia coli fda ( ts ) mutant NP315, the growth defect of the strain was complemented at temperatures >35°C. In Northern hybridisations, we identified an fda transcript of 1200 bp length. The transcript length indicates that the fda gene is transcribed from its own promoter. Attempts to isolate fda knock out mutants were not successful. Streptomyces lividans strains with a second copy of the fda gene were constructed and analysed.