Open AccessXanthomonas oryzae pv. oryzae recA is transcribed and regulated from multiple promoters
Author(s) -
Sukchawalit Rojana,
Mongkolsuk Skorn
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10579.x
Subject(s) - promoter , xanthomonas oryzae pv. oryzae , biology , xanthomonas oryzae , primer extension , genetics , xanthomonas , transcription (linguistics) , gene , gene expression , base sequence , linguistics , philosophy
Transcription regulation of Xanthomonas oryzae pv. oryzae recA was characterized. Primer extension experiments showed that recA is transcribed from three promoters designated P1, P2 and P3. The sequences of −10 and −35 regions of these promoters have moderate homology to the proposed consensus sequence for a Xanthomonas promoter. Putative SOS boxes were identified in the vicinity of P1 and P2 promoters. Deletion analysis and in vivo monitoring of promoter activity of these promoters revealed that the three promoters have different characteristics. P1 and P2 show stress‐inducible high and low promoter strengths respectively. P3 is a non‐inducible moderate promoter strength. These promoters are regulated by two SOS boxes. The multiplicity of promoters and SOS boxes provides back‐up systems to ensure proper regulation of recA .