Open AccesstRNA genes were found in Piscirickettsia salmonis 16S–23S rDNA spacer region (ITS)
Author(s) -
Casanova Andrés,
Obreque C Johanna,
Sandino G Ana Marı́a,
Jashés Matilde
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10576.x
Subject(s) - internal transcribed spacer , biology , gene , 23s ribosomal rna , polymerase chain reaction , genetics , ribosomal rna , sequence analysis , 16s ribosomal rna , spacer dna , transfer rna , ribosomal dna , microbiology and biotechnology , phylogenetics , rna , ribosome
Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S–23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF‐89 and EM‐90. Two main ITS amplification products were obtained by PCR using L1 and G1 primers, differing from that described where only one ITS region was found. By Southern blot, it was established that these two amplification products contained sequences related to P. salmonis ITS. Sequence analysis confirmed that P. salmonis had two ITS regions: ITS A and ITS B. In both isolates, the smaller (ITS B) corresponded to ITS sequences previously described for each one, and the larger (ITS A) were almost the same as their respective ITS B sequences interrupted by an insert which contained two tRNAs genes: tRNA‐Ile and tRNA‐Ala.