Open AccessCloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus
Author(s) -
Tamura Haruki,
Kikuchi Toshiaki,
Shirato Ryuichi,
Kato Hirohisa
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10573.x
Subject(s) - streptococcus sobrinus , biology , streptococcus mutans , homology (biology) , gene , peptide sequence , microbiology and biotechnology , antigen , serotype , genetics , bacteria
We have cloned and sequenced the gene encoding the surface protein antigen PAa (antigen I/II family) from Streptococcus cricetus E49 (serotype a) using degenerate PCR. The deduced amino acid sequence of PAa reveals two repeating regions (A region; alanine‐rich region, P region; proline‐rich region). Two additional tandem repeats were found in the A region and part of the P region was deleted compared to antigen I/II. Homology and phylogenetic analyses reveal that PAa is homologous to Streptococcus sobrinus PAg rather than Streptococcus mutans PAc. Using degenerate PCR a gene homologous to PAc was identified in Streptococcus intermedius , but not found in Streptococcus rattus or Streptococcus anginosus .