Open AccessModified xylE and xylTE reporter genes for use in Streptomyces : analysis of the effect of xylT
Author(s) -
GonzálezCerón Gabriela,
Licona Paula,
Servı́nGonzález Luis
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10569.x
Subject(s) - reporter gene , dioxygenase , gene , plasmid , streptomyces griseus , biology , catechol , streptomyces , promoter , streptomyces albus , microbiology and biotechnology , biochemistry , genetics , gene expression , bacteria
The reporter gene xylE (encoding catechol 2,3‐dioxygenase) has been modified for a more rational use in Streptomyces . Two reporter fragments, one containing xylE , and the other containing also the upstream gene xylT (which encodes a soluble ferredoxin), have been constructed to allow precise fusion of regulatory regions to the reporter genes. Identical fusions of these xylE and xylTE reporter fragments to the Streptomyces dagA and tipA promoters, in low and high copy number plasmids, show that the levels of xylE mRNA and catechol 2,3‐dioxygenase activities are significantly higher when xylT is present.