Open AccessPleiotropic mutants hypersensitive to heavy metals and to oxidative stress in Chlamydomonas reinhardtii
Author(s) -
Hanikenne Marc,
Matagne René Fernand,
Loppes Roland
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10549.x
Subject(s) - chlamydomonas reinhardtii , mutant , insertional mutagenesis , mutagenesis , paraquat , phenotype , biology , oxidative stress , gene , genetics , microbiology and biotechnology , chemistry , biochemistry
Abstract Insertional mutagenesis was used in Chlamydomonas reinhardtii to isolate original mutants hypersensitive to multiple drugs and physical agents. Out of 5200 transformants analyzed, 13 mutants belonging to seven phenotypic classes were isolated. Five were exclusively sensitive to cadmium and represented two loci. The other mutants were pleiotropic and presented a cross sensitivity to several (2–6) of the following agents: cadmium, copper, lead, paraquat, hydrogen peroxide, UVC and light. In all mutants analyzed, the hypersensitive phenotype was most probably due to a single mutational event. The sensitivity of several pleiotropic mutants to a broad range of physical and chemical agents suggests that the disrupted genes are involved in multiple stress responses.