Open AccessStable carbon isotope fractionations of the hyperthermophilic crenarchaeon Metallosphaera sedula
Author(s) -
Meer Marcel T.J.,
Schouten Stefan,
Rijpstra W.Irene C.,
Fuchs Georg,
Sinninghe Damsté Jaap S.
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10542.x
Subject(s) - isotopes of carbon , carbon fibers , chemistry , environmental chemistry , computer science , composite number , total organic carbon , algorithm
The stable carbon isotopic compositions of the inorganic carbon source, bulk cell material, and isoprenoid lipids of the hyperthermophilic crenarchaeon Metallosphaera sedula , which uses a 3‐hydroxypropionate‐like pathway for autotrophic carbon fixation, have been measured. Bulk cell material was approximately 3‰ enriched in 13 C relative to the dissolved inorganic carbon, and 2‰ depleted in 13 C relative to isoprenoid membrane lipids. The isotope data suggested that M. sedula uses mainly bicarbonate rather than CO 2 as inorganic carbon source, which is in accordance with a 3‐hydroxypropionate‐like carbon fixation pathway. To the best of our knowledge this is the first report of 13 C fractionation effects of such a hyperthermophilic crenarchaeon.