Open AccessIsolation and diagnostic potential of IS Mav2 , a novel insertion sequence‐like element from Mycobacterium avium subspecies paratuberculosis
Author(s) -
Strommenger Birgit,
Stevenson Karen,
Gerlach GeraldF
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10536.x
Subject(s) - paratuberculosis , mycobacterium avium subspecies paratuberculosis , biology , insertion sequence , mycobacterium , microbiology and biotechnology , transposable element , sequence analysis , streptomyces coelicolor , rhodococcus equi , genome , virology , genetics , dna , bacteria , streptomyces , gene , virulence
A novel Mycobacterium avium ssp. paratuberculosis ( M. paratuberculosis ) specific insertion sequence has been identified by representational difference analysis and designated as IS Mav2 . IS Mav2 has no similarity to known mycobacterial IS elements but shows more than 50% identity to a non‐composite transposon of Streptomyces coelicolor at the DNA and protein level. IS Mav2 is present in at least three copies on the genome as assessed by Southern blot analysis and its potential value as a diagnostic tool was confirmed by PCR analyses on 79 M. paratuberculosis field isolates, nine M. avium ssp. avium isolates, and the reference strains of nine other mycobacterial species.