Open AccessDirected immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose‐binding domain
Author(s) -
Lehtiö Janne,
Wernérus Henrik,
Samuelson Patrik,
Teeri Tuula T,
Ståhl Stefan
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10521.x
Subject(s) - recombinant dna , trichoderma reesei , cellulose , cellulase , monoclonal antibody , chemistry , biochemistry , microbiology and biotechnology , biology , antibody , gene , immunology
The immobilization of recombinant staphylococci onto cellulose fibers through surface display of a fungal cellulose‐binding domain (CBD) was investigated. Chimeric proteins containing the CBD from Trichoderma reesei cellulase Cel6A were found to be correctly targeted to the cell wall of Staphylococcus carnosus cells, since full‐length proteins could be extracted and affinity‐purified. Furthermore, surface accessibility of the CBD was verified using a monoclonal antibody and functionality in terms of cellulose‐binding was demonstrated in two different assays in which recombinant staphylococci were found to efficiently bind to cotton fibers. The implications of this strategy of directed immobilization for the generation of whole‐cell microbial tools for different applications will be discussed.