Open AccessCloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isi B and its own promoter
Author(s) -
Bes M.Teresa,
Hernández José Angel,
Peleato M.Luisa,
Fillat María F
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb09467.x
Subject(s) - anabaena , biology , open reading frame , promoter , gene , recombinant dna , escherichia coli , cyanobacteria , biochemistry , cloning (programming) , synechococcus , molecular cloning , microbiology and biotechnology , homology (biology) , dna binding protein , peptide sequence , genetics , bacteria , gene expression , transcription factor , computer science , programming language
A gene coding for a Fur (ferric uptake regulation) protein from the cyanobacterium Anabaena PCC 7119 has been cloned and overexpressed in Escherichia coli . DNA sequence analysis confirmed the presence of a 151‐amino‐acid open reading frame that showed homology with the Fur proteins reported for the unicellular cyanobacteria Synechococcus 7942 and Synechocystis PCC 6803. Two putative Fur‐binding sites were detected in the promoter regions of the fur gene from Anabaena . Partially purified recombinant Fur binds to the flavodoxin promoter as well as its own promoter. This suggests that the Fur gene is autoregulated in Anabaena .