Open AccessA Mycobacterium smegmatis gyrase B specific monoclonal antibody reveals association of gyrase A and B subunits in the cell
Author(s) -
Manjunatha U.H.,
Somesh B.P.,
Nagaraja V.,
Visweswariah Sandhya S.
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb09451.x
Subject(s) - dna gyrase , mycobacterium smegmatis , novobiocin , microbiology and biotechnology , biology , protein subunit , epitope , dna , monoclonal antibody , escherichia coli , immunoprecipitation , genetics , antibody , gene , mycobacterium tuberculosis , bacteria , medicine , tuberculosis , pathology
DNA gyrase is a unique topoisomerase, which plays important roles in macromolecular events like DNA replication, transcription and genetic recombination. In this study a high affinity monoclonal antibody to the gyrase B (GyrB) subunit of Mycobacterium smegmatis was characterized, which did not cross‐react with either the Escherichia coli GyrB subunit or with GyrB subunits from other mycobacterial species. The antibody recognized an epitope in the N‐terminus, novobiocin‐binding domain of GyrB. Immunoprecipitation of gyrase from M. smegmatis cell lysate revealed an association, mediated by ionic interactions, of gyrase A and GyrB subunits in the cell. This antibody is a valuable tool for structure–function analysis and immunocytological studies of mycobacterial DNA gyrase.