Open AccessNovel method for the proteomic investigation of a dairy‐associated Bacillus cereus biofilm
Author(s) -
Oosthuizen Marinda C.,
Steyn Bridgitta,
Lindsay Denise,
Brözel Volker S.,
Holy Alexander
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb09444.x
Subject(s) - bacillus cereus , biofilm , cereus , proteome , glass wool , microbiology and biotechnology , strain (injury) , chemistry , biology , bacteria , food science , biochemistry , genetics , organic chemistry , layer (electronics) , anatomy
The biofilm proteome of a dairy‐associated Bacillus cereus strain ( B. cereus 5) was investigated. Biofilm biomass of sufficient concentration for 2D‐PAGE was obtained by growing the culture in the presence of glass wool. B. cereus 5 readily attached to the glass wool and biofilms formed within 18 h. The biofilm proteome of whole‐cell proteins revealed that 10 proteins were synthesized as a result of surface attachment of which four were unique to the biofilm profile. Seven proteins appeared to be absent in the biofilm profile. The altered proteomes indicated that changes took place in the regulation of protein expression when B. cereus 5 cells attached to surfaces.