Open AccessDynamic accumulation and degradation of poly(3‐hydroxyalkanoate)s in living cells of Azotobacter vinelandii UWD characterized by 13 C NMR
Author(s) -
Yan YongBin,
Wu Qiong,
Zhang RiQing
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09435.x
Subject(s) - azotobacter vinelandii , azotobacteraceae , degradation (telecommunications) , chemistry , carbon 13 nmr , strain (injury) , industrial fermentation , monomer , biochemistry , nuclear chemistry , biology , organic chemistry , fermentation , polymer , nitrogen , nitrogenase , telecommunications , nitrogen fixation , computer science , anatomy
The synthesis and degradation of poly(3‐hydroxybutyrate) (PHB) and poly(3‐hydroxybutyrate‐ co ‐hydroxyvalerate) (P(HB‐ co ‐HV)) by Azotobacter vinelandii UWD were investigated using natural abundance solution 13 C nuclear magnetic resonance (NMR) in vivo in shake flask culture and in fermenter culture. The synthesis and the degradation of poly(3‐hydroxyalkanoate)s (PHA) monomers hydroxybutyrate (HB) and hydroxyvalerate (HV) had different rates. The amount of HB and HV increased dramatically in the initial degradation stage. The results suggest that the intracellular PHA of strain UWD was the subject of dynamic metabolic processing. 13 C NMR in vivo analysis provided a rapid, easy, accurate, non‐destructive method to obtain valuable information on the metabolism of PHA.