Open AccessEnzymatic versus spontaneous S ‐methyl thioester synthesis in Geotrichum candidum
Author(s) -
Helinck Sandra,
Spinnler Henry E,
Parayre Sandrine,
DameCahagne Michèle,
Bonnarme Pascal
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09430.x
Subject(s) - geotrichum , thioester , methanethiol , chemistry , enzyme , residue (chemistry) , stereochemistry , fatty acid , biochemistry , biosynthesis , organic chemistry , sulfur , food science
The synthesis of short chain S ‐methyl thioesters was investigated in Geotrichum candidum strain GcG. The results indicated the involvement of an enzymatic reaction in this microorganism that led to the synthesis of S ‐methyl thioacetate (MTA) when methanethiol and acetyl‐CoA were used as substrates. MTA was generated from these substrates by enzymatic or spontaneous reactions, whose relative importance depended largely on pH and temperature. For longer chain acyl‐CoA compounds (C3 to C6), thioester synthesis was primarily spontaneous. Short chain fatty acid activation by a CoA residue probably is a prerequisite for the synthesis of S ‐methyl thioesters.