Open AccessIdentification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles
Author(s) -
On Stephen L.W,
Harrington Clare S
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09419.x
Subject(s) - campylobacter , amplified fragment length polymorphism , subspecies , biology , campylobacter jejuni , microbiology and biotechnology , dendrogram , numerical taxonomy , phylogenetic tree , genetics , bacteria , zoology , taxonomy (biology) , genetic diversity , population , gene , demography , sociology
Amplified fragment length polymorphism (AFLP)‐based profiling was performed on 138 strains representing all named Campylobacter species and subspecies. Profiles of 15/16 species comprised 6 to greater than 100 fragments and were subjected to numerical analysis. The mean similarity of 48 duplicate, outbreak and/or ‘identical’ strain profiles exceeded 94%. Species were clearly distinguished at the 17.90% similarity (S‐) level in the dendrogram. Subspecies of Campylobacter jejuni and Campylobacter hyointestinalis , and biovars of Campylobacter lari and Campylobacter sputorum were distinguished at higher S‐levels. All outbreak or ‘genetically identical’ strains of C. jejuni subsp. jejuni , Campylobacter coli , C. hyointestinalis and C. sputorum clustered at S‐levels >92% and were distinguished from unrelated strains. Numerical analysis of AFLP profiles is useful for concurrent identification of taxonomic and epidemiological relationships among most Campylobacter species.