Open AccessCharacterization of wine yeasts by temperature gradient gel electrophoresis (TGGE)
Author(s) -
HernánGómez S.,
Espinosa J.C.,
Ubeda J.F.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09400.x
Subject(s) - temperature gradient gel electrophoresis , heteroduplex , wine , biology , gel electrophoresis , yeast , intraspecific competition , 16s ribosomal rna , chromatography , microbiology and biotechnology , genetics , bacteria , gene , chemistry , food science , ecology
18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtained. Heteroduplex analysis was capable of differentiating between similar bands produced by two different species, thereby enhancing the resolution of the TGGE, yielding valuable information in a short time without the need of sequencing or complicated equipment.