Open AccessIsolation of a new insertion element of Yersinia intermedia closely related to remnants of mobile genetic elements present on Yersinia plasmids harboring the Yop virulon
Author(s) -
Strauch Eckhard,
Hoffmann Bernd,
Heins Gudrun,
Appel Bernd
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09399.x
Subject(s) - plasmid , yersinia enterocolitica , insertion sequence , yersinia , biology , mobile genetic elements , genetics , transposable element , kanamycin , transposition (logic) , transposase , gene , inverted repeat , microbiology and biotechnology , direct repeat , yersinia pseudotuberculosis , virulence , genome , bacteria , linguistics , philosophy
A new insertion element present in two alleles, designated IS 1635.1 and IS 1635.2 , was identified on a plasmid of a Yersinia intermedia strain by hybridization with the Yersinia enterocolitica pYV virulence plasmid. IS 1635.1 and IS 1635.2 are 861 bp long, carry imperfect inverted terminal repeats and possess a single open reading frame encoding a putative transposase of the IS 6 family. A truncated IS 1635 element is present immediately downstream of element IS 1635.2 . The capacity of the IS 1635 elements to mediate transposition in Yersinia was demonstrated with a R6K‐derived suicide vector, where a kanamycin resistance gene had been inserted between IS 1635.1 and IS 1635.2 . Hybridization and sequence alignments showed that remnants of IS 1635 ‐like insertion elements harboring large deletions and point mutations are present on the Yop virulon harboring plasmids of pathogenic Yersinia strains. In a few cases, the IS 1635 element has also been found on plasmids of apathogenic Yersinia strains.