Open AccessCharacterisation of a Pasteurella multocida esterase gene which confers a hemolytic phenotype in Escherichia coli under anaerobic conditions
Author(s) -
Hunt Meredith L.,
Cox Angela J.,
Ruffolo Carmel G.,
Rajakumar Kumar,
Adler Ben
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09390.x
Subject(s) - pasteurella multocida , escherichia coli , microbiology and biotechnology , biology , cytolysin , hemolysis , virulence , esterase , anaerobic exercise , phenotype , pasteurellosis , mutant , enterobacteriaceae , gene , bacteria , genetics , enzyme , biochemistry , immunology , physiology
Investigation of the hemolytic phenotype under anaerobic growth conditions of an avian Pasteurella multocida strain, PBA100, resulted in the identification and characterisation of a gene encoding an esterase enzyme, mesA , that conferred a hemolytic phenotype in Escherichia coli under anaerobic conditions. MesA appeared to be expressed and functional under anaerobic and aerobic conditions in both E. coli and P. multocida . A P. multocida mesA mutant was generated which resulted in the loss of acetyl esterase activity under anaerobic conditions. However, this mutation did not cause any attenuation of virulence for mice nor a detectable change to the anaerobic hemolytic phenotype of P. multocida . In E. coli MesA appeared to cause hemolysis indirectly by the induction of the latent E. coli K‐12 cytolysin, sheA .