Open AccessA unique and common restriction fragment pattern of the nucleotide sequences homologous to the genome of Vf33, a filamentous bacteriophage, in pandemic strains of Vibrio parahaemolyticus O3:K6 O4:K68, and O1:K untypeable
Author(s) -
Chang Bin,
Yoshida Shinichi,
Miyamoto Hiroshi,
Ogawa Midori,
Horikawa Kazumi,
Ogata Kikuyo,
Nishibuchi Mitsuaki,
Taniguchi Hatsumi
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09387.x
Subject(s) - biology , vibrio parahaemolyticus , southern blot , genome , restriction fragment length polymorphism , genetics , bacteriophage , virology , lysogenic cycle , gene , filamentous bacteriophage , restriction fragment , microbiology and biotechnology , bacteria , polymerase chain reaction , escherichia coli
Vf33 is a filamentous bacteriophage isolated from Vibrio parahaemolyticus . We performed Southern blot hybridization analysis to examine the distribution of Vf33‐related genetic elements in the pandemic strains (O3:K6 strains isolated between 1995 and 1997, O4:K68 and O1:K untypeable strains isolated between 1997 and 1999) of V. parahaemolyticus . Nucleotide sequences homologous to the Vf33 DNA were detected in all 57 test strains including pandemic and non‐pandemic strains. However, the profiles of hybridization, including the restriction fragment length polymorphism, with nine Vf33‐derived DNA probes exhibited by the pandemic strains were identical and were different from those by the non‐pandemic strains. The results support the hypothesis that the pandemic strains are clonal, and suggest a possibility that they have acquired (a) new gene(s) via a Vf33‐like filamentous phage.