Open AccessGenetic variations among Mycoplasma bovis strains isolated from Danish cattle
Author(s) -
Kusiluka Lughano J.M.,
Kokotovic Branko,
Ojeniyi Bente,
Friis Niels F.,
Ahrens Peter
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09368.x
Subject(s) - danish , biology , microbiology and biotechnology , mycoplasma , philosophy , linguistics
The genetic heterogeneity of Mycoplasma bovis strains isolated in Denmark over a 17‐year period was investigated. Forty‐two field strains isolated from different geographic locations and specimens, including strains from 21 herds involved in two outbreaks of M. bovis ‐induced mastitis, and the type strain of M. bovis (PG45 T ) were assayed for variations in the Bgl II and Mfe I restriction sites in the chromosomal DNA by using the amplified fragment length polymorphism (AFLP) fingerprinting technique. The obtained genomic fingerprints consisted of 62–68 AFLP fragments in the size range of 50–500 bp. Among the analyzed strains, 18 different AFLP profiles were detected. The similarity between individual fingerprints, calculated by Dice similarity coefficient, ranged from 0.9 to 1.0. Twenty‐five strains, including 23 which were isolated during two outbreaks of M. bovis ‐induced mastitis which occurred 2 years apart, showed indistinguishable AFLP patterns. More genetic diversity was observed among the recent strains. The similarity of the genotypes of the field strains to that of the M. bovis type strain (PG45 T ) was 97.7%. The results of this study have demonstrated a remarkable genomic homogeneity of Danish strains of M. bovis that were probably epidemiologically related and which have remained stable for a considerable length of time. Furthermore, this study has demonstrated that AFLP can be used for genomic fingerprinting and discrimination of M. bovis strains.