Open AccessEffect of Enterocin CRL35 on Listeria monocytogenes cell membrane
Author(s) -
Minahk Carlos J.,
Farías María E.,
Sesma Fernando,
Morero Roberto D.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09362.x
Subject(s) - listeria monocytogenes , listeria , chemistry , bacteria , cell membrane , food science , microbiology and biotechnology , cell , biology , biochemistry , genetics
The antimicrobial peptide Enterocin CRL35, a class II bacteriocin, produces at high concentrations (8 μg ml −1 ) localized holes in the wall and cellular membrane of Listeria monocytogenes , reflected in the efflux of macromolecules such as proteins and other ultraviolet‐absorbing materials. At lower concentrations (0.5 μg ml −1 ), neither ultra structural changes nor macromolecules efflux were observed, however potassium and phosphate ions were released, dissipating the proton motive force. As a result the bacteria were killed.