Open AccessStructural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus
Author(s) -
Maeda Toshimichi,
Takada Narumichi,
Furushita Manabu,
Shiba Tsuneo
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09361.x
Subject(s) - vibrio parahaemolyticus , 23s ribosomal rna , biology , intergenic region , ribosomal rna , 16s ribosomal rna , microbiology and biotechnology , ribosomal intergenic spacer analysis , bacteria , genetics , gene , rna , genome , internal transcribed spacer , ribosome
The 16S–23S rRNA intergenic spacers (IGS) of Vibrio parahaemolyticus were PCR‐amplified and cloned with pT7Blue T vector. A total of six clones were isolated dependent on size difference. The clones were different with respect to both the number and the composition of the tRNA genes included, and were designated IGS‐0, IGS‐E, IGS‐IA, IGS‐AE, IGS‐EKV and IGS‐EKAV. IGS‐EKAV included the cluster of tRNA Glu ‐tRNA Lys ‐tRNA Ala ‐tRNA Val ; IGS‐EKV, tRNA Glu ‐tRNA Lys ‐tRNA Val ; IGS‐AE, tRNA Ala ‐tRNA Glu ; IGS‐IA, tRNA Ile ‐tRNA Ala ; and IGS‐E, tRNA Glu . IGS‐0 had no tRNA gene. Some similarities were found in the nucleotide sequence of the non‐coding regions flanked by the tRNA genes. The structure difference found in the spacers is meaningful for elucidating the evolutionary line of each ribosomal RNA operon and the profile is applicable as a molecular marker of the bacterium.