Open AccessDistribution of ecto 5′‐nucleotidase on Mycoplasma species associated with arthritis
Author(s) -
Johnson Sheena,
Pitcher David
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09359.x
Subject(s) - 5' nucleotidase , biology , microbiology and biotechnology , distribution (mathematics) , arthritis , immunology , biochemistry , mathematics , enzyme , mathematical analysis
The enzyme ecto 5′‐nucleotidase (5′N) was found to be active on 8/14 strains of Mycoplasma fermentans , K m (±S.D.) 3.8±2.8 μM 5′‐AMP, and on the type strain of Mycoplasma pulmonis , K m 0.63 μM 5′‐AMP. The six M. fermentans strains lacking 5′N activity were related by restriction fragment length polymorphism typing. At pH 8.5, the type strains of Mycoplasma arthritidis , Mycoplasma buccale and Ureaplasma urealyticum showed a relatively non‐specific phosphatase activity against 5′‐AMP but no activity was shown by the type strains of Mycoplasma genitalium , Mycoplasma hominis , Mycoplasma orale , Mycoplasma penetrans , Mycoplasma pneumoniae and Mycoplasma salivarium at this pH. M. fermentans has been reported from rheumatoid joints, which show a raised 5′N activity on their synovial cells and in their fluid which may be associated directly or indirectly with the mycoplasma.