Open AccessThe yabG gene of Bacillus subtilis encodes a sporulation specific protease which is involved in the processing of several spore coat proteins
Author(s) -
Takamatsu Hiromu,
Imamura Atsuo,
Kodama Takeko,
Asai Kei,
Ogasawara Naotake,
Watabe Kazuhito
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09355.x
Subject(s) - bacillus subtilis , proteolysis , biology , protease , spore , mutant , recombinant dna , escherichia coli , bacillaceae , biochemistry , subtilisin , microbiology and biotechnology , gene , enzyme , bacteria , genetics
The synthesis and proteolysis of the spore coat proteins, SpoIVA and YrbA, of Bacillus subtilis were analyzed using antisera. Almost no intact full‐length proteins of either type were extracted from wild‐type spores, while yabG mutant spores contained intact SpoIVA and YrbA proteins. We purified recombinant YrbA and YabG proteins from Escherichia coli transformants and found that YrbA was cleaved to the smaller moiety in the presence of YabG in vitro. These observations indicate that YabG is a protease involved in the proteolysis and maturation of SpoIVA and YrbA proteins, conserved with the cortex and/or coat assembly by B. subtilis .