Open AccessThe NAD‐glycohydrolase ( nga ) gene of Streptococcus pyogenes
Author(s) -
Ajdic Dragana,
McShan William M.,
Savic Dragutin J.,
Gerlach Dieter,
Ferretti Joseph J.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09345.x
Subject(s) - streptococcus pyogenes , streptolysin , nad+ kinase , gene , microbiology and biotechnology , biology , streptococcus , chemistry , bacteria , genetics , biochemistry , enzyme , staphylococcus aureus , bacterial protein
The gene for NAD‐glycohydrolase ( nga ) of group A streptococci (Streptococcus pyogenes ) was identified and shown to be located immediately adjacent to the gene for streptolysin O ( slo ). The nga gene contains 1341 base pairs and encodes a protein of 447 amino acids, including an N‐terminal signal peptide. Results from analysis with the polymerase chain reaction indicated that the nga gene is present in all of the strains tested. Functional extracellular NAD‐glycohydrolase, also known as NADase, was detected among a wide variety of clinical isolates and known laboratory strains and shown to be present in 72% of 100 strains examined. In contrast, 92% of strains isolated from patients with invasive streptococcal infections were positive for NADase production.