Open AccessIdentification of DNA amplifications near the center of the Streptomyces coelicolor M145 chromosome
Author(s) -
Redenbach Matthias,
Kleinert Eveline,
Stoll Alexander
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09328.x
Subject(s) - streptomyces coelicolor , dna , chromosome , biology , genetics , microbiology and biotechnology , streptomyces , circular bacterial chromosome , gene , dna replication , bacteria
Linear streptomycete chromosomes frequently undergo spontaneous gross DNA rearrangements at the terminal regions. Large DNA deletions of the chromosome ends are in many cases associated with tandemly reiterated DNA amplifications, found at the border of the deletable areas. In contrast to previous reports, we have discovered amplifications near the center of the Streptomyces coelicolor M145 chromosome. The detected amplified units of DNA are 19.9 kb and 16 kb in length and exist in copy numbers of 30 and 40, respectively. Both amplifications were located in the same region and share at least 3.6 kb.