Open AccessPeptide mimics elicit antibody responses against the outer‐membrane lipooligosaccharide of group B Neisseria meningitidis
Author(s) -
Charalambous Bambos M.,
Feavers Ian M.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09317.x
Subject(s) - neisseria meningitidis , toxoid , microbiology and biotechnology , antibody , diphtheria toxin , immune system , neisseriaceae , bacterial outer membrane , peptide , monoclonal antibody , biology , meningococcal vaccine , immunoglobulin g , immunology , antibiotics , immunization , toxin , bacteria , escherichia coli , biochemistry , genetics , gene
As an alternative approach towards the development of a meningococcal vaccine, the potential of peptide mimics of lipooligosaccharide (LOS) to elicit cross‐reactive immune responses against LOS was investigated. The heptapeptides SMYGSYN and APARQLP were identified by enrichment from a coliphage display library with a LOS‐specific monoclonal antibody. Mice immunised with these peptides conjugated to diphtheria toxoid elicited a total IgG response to LOS with geometric mean titres 2–4 times higher compared with non‐immunised controls. There was an increase in LOS‐specific IgG1 immunoglobulin, whereas specific IgG2a and IgG3 decreased slightly in response to immunisation. The data demonstrated that peptide mimics can elicit immune responses against meningococcal LOS.