Open AccessA Lactobacillus helveticus plasmid detects restriction fragment length polymorphism in different bacterial species
Author(s) -
Gancheva Anna G.,
Miteva Vanya I.,
Stefanova Tsona T.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09308.x
Subject(s) - plasmid , biology , restriction fragment length polymorphism , lactobacillus helveticus , genetics , terminal restriction fragment length polymorphism , restriction fragment , dna , microbiology and biotechnology , lactobacillus , polymerase chain reaction , gene , bacteria
A small cryptic Lactobacillus helveticus plasmid, pLBL4, was able to reveal restriction fragment length polymorphism in different bacterial species including Lactobacillus species, Bacillus species, and Escherichia coli when used as a DNA probe. The observed polymorphism was a result of the combined hybridization of several microsatellite sequences. The 6‐bp sequence (TTGTTT) was repeated 12 times, seven of which were concentrated within the region between 1791 and 1997 bp of the plasmid sequence. The polymorphic patterns generated with pLBL4 differed from those obtained with M13 DNA in the larger number of bands observed. The results presented here open the possibility of using pLBL4 as a new broad‐spectrum polymorphic DNA probe for fingerprint analysis.