Open AccessBinding of catalase by Gardnerella vaginalis
Author(s) -
Jarosik Gregory P.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09285.x
Subject(s) - catalase , gardnerella vaginalis , western blot , microbiology and biotechnology , blot , trypsin , biology , enzyme , dot blot , chemistry , biochemistry , dna , bacterial vaginosis , gene
Previous work has demonstrated that Gardnerella vaginalis can utilize catalase as a sole source of iron. In this study, the interaction between G. vaginalis cells and catalase was investigated. G. vaginalis cells were shown to bind digoxigenin (DIG)‐labeled catalase using a solid phase dot blot assay. An increase in catalase binding was observed from cells grown under iron‐restrictive conditions. Western blot analysis of G. vaginalis proteins resulted in the detection of a putative catalase‐binding protein with an estimated molecular mass of 128 kDa. The 128‐kDa catalase‐binding protein was not detected from intact G. vaginalis cells treated with trypsin prior to Western blot analysis suggesting this protein may be surface‐exposed.