Open AccessExpression of the tetA (C) tetracycline efflux pump in Escherichia coli confers osmotic sensitivity
Author(s) -
Stavropoulos Tom A.,
Strathdee Craig A.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09277.x
Subject(s) - tetracycline , escherichia coli , efflux , osmotic shock , aminoglycoside , biology , microbiology and biotechnology , chemistry , biochemistry , antibiotics , gene
Expression of tetA (C) in Escherichia coli confers resistance to tetracycline as well as sensitivity to nickel and cadmium salts, lipophilic chelating agents, and aminoglycoside antibiotics. In this report we determine that high‐level expression of tetA (C) also confers an osmotic sensitivity. The osmotic‐sensitive phenotype is distinct from the tetracycline‐resistant phenotype and can be localized to a domain contained within the first 98 amino acid residues of the TetA(C) polypeptide.