Open AccessComparison of global transcription responses allows identification of Vibrio cholerae genes differentially expressed following infection
Author(s) -
Das Soumita,
Chakrabortty Amit,
Banerjee Rajat,
Roychoudhury Susanta,
Chaudhuri Keya
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09267.x
Subject(s) - vibrio cholerae , biology , gene , enhancer , genetics , transcription (linguistics) , transcription factor , in vivo , bacteria , linguistics , philosophy
Comparison of global transcription profiles of Vibrio cholerae grown in vitro and in vivo revealed that 20% of the genome was repressed and about 5% was induced under in vivo conditions. Hybridization with the cloned genes revealed that the virulence genes ctx, toxR, toxT and tcpA were induced under in vivo conditions. Dissection of two in vivo induced cosmids identified another set of three genes homologous to cheY1 involved in motility and chemotaxis, pnu C encoding the major component of the nicotinamide mononucleotide transport system and icmF belonging to a cassette involved in multiplication inside host cells. These results demonstrate that the global transcription profile approach might be a powerful method for identification of differentially expressed transcripts under in vivo conditions.