Open AccessComparison of three serological methods for detection of Lewis antigens on the surface of Helicobacter pylori
Author(s) -
Hynes Sean O,
Moran Anthony P
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09264.x
Subject(s) - serology , helicobacter pylori , antigen , microbiology and biotechnology , helicobacter , biology , virology , immunology , antibody , genetics
Chemical studies have shown that lipopolysaccharide (LPS) from certain strains of Helicobacter pylori expresses the Lewis (Le) blood group antigens, predominantly Le x and/or Le y . Serological methods have been used in a number of studies to assess the level of Le expression by H. pylori . However, serological and chemical analyses of a strain's LPS do not always agree. The purpose of this study was to evaluate and compare serological methods used to detect the expression of these antigens by H. pylori . A set of 34 Irish clinical isolates and three culture collection strains had their Le antigen expression assessed using three serological methods, serodot, Western blotting and enzyme‐linked immunosorbent assay. A combination of all three methods was used to estimate Le x (59%) and Le y (94%) expression and this may allow a more accurate assessment of Le expression by isolates for pathogenesis studies.