Open AccessCloning and sequencing analysis of TRP1 gene of Flammulina velutipes
Author(s) -
Nakai Ryohsuke,
Sen Kikuo,
Kurosawa Shinichi,
Shibai Hiroshiro
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09261.x
Subject(s) - glutamine amidotransferase , complementation , biochemistry , phanerochaete , biology , microbiology and biotechnology , gene , chemistry , genetics , glutamine , enzyme , amino acid , mutant
The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole‐3‐glycerol phosphate synthase) and N ‐(5′‐phosphoribosyl) anthranilate isomerase, in order of NH 2 ‐glutamine amidotransferase–indole‐3‐glycerol phosphate synthase– N ‐(5′‐phosphoribosyl) anthranilate isomerase–COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC .