Open AccessReplication origin of Streptococcus pyogenes , organization and cloning in heterologous systems
Author(s) -
Suvorov Alexander N.,
Ferretti Joseph J.
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09246.x
Subject(s) - streptococcus pyogenes , plasmid , biology , origin of replication , transformation (genetics) , bacillus subtilis , genetics , escherichia coli , cloning (programming) , streptococcus , dna , microbiology and biotechnology , bacteria , gene , staphylococcus aureus , computer science , programming language
The origin of DNA replication ( oriC ) of Streptococcus pyogenes , group A streptococci (GAS), has been cloned in Escherichia coli and reintroduced by transformation into other GAS strains. Transformation frequencies into GAS strains with oriC ‐carrying plasmids occurred with unusually high frequencies. However, the oriC ‐containing plasmids in the new recipients were found to be unstable and had a tendency to integrate into the chromosome, even when a recA GAS strain was used as a recipient. The GAS oriC was able to direct the replication of autonomous plasmids in group B streptococcal recipients. The chromosomal organization of the oriC region of GAS relative to other bacterial species appears to be similar to oriC of Bacillus subtilis and other Gram‐positive microorganisms.