Open AccessPartial characterization of a genomic island associated with the multidrug resistance region of Salmonella enterica Typhymurium DT104
Author(s) -
Boyd David A,
Peters Geoffrey A,
Ng LaiKing,
Mulvey Michael R
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09245.x
Subject(s) - prophage , salmonella enterica , genomic island , genetics , biology , integrase , genome , gene , open reading frame , direct repeat , salmonella , sequence analysis , inverted repeat , insertion sequence , multiple drug resistance , homology (biology) , whole genome sequencing , escherichia coli , drug resistance , bacteriophage , peptide sequence , transposable element , bacteria
This study describes the identification of the insertion site and partial characterization of a 43‐kb region harboring the genes associated with the penta‐resistant phenotype of a Canadian isolate of Salmonella enterica Typhymurium DT104 labelled 96‐5227. The 43‐kb fragment, here referred to as Salmonella genomic island I (SgiI), was found in the genome of S. enterica Typhymurium between the thdf and a prophage CP‐4‐like integrase ( int2 ) gene and is flanked by an imperfect 18‐bp direct repeat. A region downstream of sul I in the right end of SgiI contained four open reading frames which includes an IS 6100 element, and a 2‐kb region from the left end contained two open reading frames which showed homology to an integrase and an excisionase. Furthermore, a 1.9‐kb retron sequence located between int2 and yidY was identified which may be unique to the S. enterica Typhymurium genome. The int ‐retron sequence is flanked by a 27‐bp imperfect direct repeat.