Open AccessMultiplex PCR for the identification of white Tuber species
Author(s) -
Amicucci Antonella,
Guidi Chiara,
Zambonelli Alessandra,
Potenza Lucia,
Stocchi Vilberto
Publication year - 2000
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2000.tb09241.x
Subject(s) - truffle , biology , primer (cosmetics) , multiplex polymerase chain reaction , fungus , white (mutation) , internal transcribed spacer , polymerase chain reaction , multiplex , botany , ascomycota , specific identification , genetics , gene , chemistry , ribosomal rna , organic chemistry
Species‐specific primers selected from the internal transcribed spacer region sequence were used to set up a multiplex polymerase chain reaction (PCR) able to simultaneously identify the white truffle species Tuber magnatum , Tuber borchii , Tuber maculatum and Tuber puberulum . Furthermore, a primer specific for the competitive fungus Sphaerosporella brunnea was designed and added to the multiplex PCR set, allowing the detection of the Tuber species and the contaminant fungus in a one‐step reaction.